Genotyping and study of the pauA and sua genes of Streptococcus uberis isolates from bovine mastitis

PERRIG MELINA; AMBROGGIOA MARÍA; BUZZOLA FERNANDA; MARCIPAR IVÁN; CALVINHO LUIS; VEAUTE CAROLINA; BARBAGELATA MS

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2015 vol. 47 p. 282 - 294

0325-7541

This study aimed to determine the clonal relationship among 137 Streptococcusuberis isolates from bovine milk with subclinical or clinical mastitis in Argentina and to assessthe prevalence and conservation of pauA and sua genes. This information is critical for therational design of a vaccine for the prevention of bovine mastitis caused by S. uberis. Theisolates were typed by random amplified polymorphic DNA (RAPD) analysis and by pulsed-fieldgel electrophoresis (PFGE). The 137 isolates exhibited 61 different PFGE types and 25 distinctRAPD profiles. Simpson?s diversity index was calculated both for PFGE (0.983) and for RAPD(0.941), showing a high discriminatory power in both techniques. The analysis of the relationshipbetween pairs of isolates showed 92.6 % concordance between both techniques indicatingthat any given pair of isolates distinguished by one method tended to be distinguished by theother. The prevalence of the sua and pauA genes was 97.8 % (134/137) and 94.9 % (130/137),respectively. Nucleotide and amino acid sequences of the sua and pauA genes from 20 S. uberisselected isolates, based on their PFGE and RAPD types and geographical origin, showed anidentity between 95 % and 100 % with respect to all reference sequences registered in GenBank.These results demonstrate that, in spite of S. uberis clonal diversity, the sua and pauA genesare prevalent and highly conserved, showing their importance to be included in future vaccinestudies to prevent S. uberis bovine mastitis.