INVESTIGADORES
ESTEIN Silvia Marcela
artículos
Título:
Immunogenicity of a chimeric subcellular vaccine in ovine brucellosis
Autor/es:
SILVIA. M ESTEIN, MARÍA A FIORENTINO, FERNANDO A. PAOLICCHI, JULIANA CASSATARO, GUILLERMO GIAMBARTOLOMEI, VANESA ZYLBERMAN, CARLOS A. FOSSATI, FERNANDO A. GOLDBAUM
Revista:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam, Holanda; Año: 2009 vol. 128 p. 227 - 228
ISSN:
0165-2427
Resumen:
Brucella ovis causes an infectious disease in sheep characterized by epididymitis and infertility in rams and
abortion in ewes. Vaccination programs are the only viable means for the control of B. ovis in countries with a high incidence.
B. melitensis Rev.1 is considered the best vaccine for the prophylaxis of ovine brucellosis but has important disadvantages.
Accordingly, research is under way to develop effective subcellular vaccines. Detergent-extracted recombinant
Omp31 from B. melitensis was previously identified as a protective immunogen against B. ovis in rams. Moreover,
our previous results demonstrate that a chimera of Brucella lumazine synthase (BLS) that contains an
immunodominant epitope of Omp31 delivered as a protein (BLS-Omp31) or plasmidic DNA vaccine (pCIbls-Omp31)
conferred protection against B. ovis infection in mice. In this work, we evaluated the immunogenicity of these vaccines
in rams using different strategies of immunisation. Seventy rams 45-month-old were randomly distributed
in following groups of immunisation: (G1) BLS-Omp31 in Freunds incomplete adjuvant (FIA), (G2) BLS-Omp31 in
QUIL A adjuvant, (G3) pCIbls-omp31 with electroporation (INOVIO, Norway), (G4) pCIbls-omp31 without electroporation,
(G5) Prime-boost, (G6) HS (hot saline) extract in FIA, (G7) PBS. Animals were vaccinated three times, four
weeks apart. G5 received four injections (DNA primeprotein boost). Rams were bled after each immunization.
Antibody responses in serum were evaluated in indirect ELISA against BLS-Omp31. Immunisation with the protein
chimera formulated with adjuvant induced IgG specific antibodies significantly higher than chimerical DNA vaccine.
However, humoral immune responsewas enhanced in electroporated sheep. Combination of a plasmidDNA priming
step followed by a boost with the homologous protein resulted in improved humoral antigen-specific response.
These results indicate that the chimerical subunit vaccine was immunogenic in rams and would be considered as
potential vaccine in ovine brucellosis.