Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress

PRYMACZOK, N.; PASQUALINO, V.M.; VIAU, V.E.; RODRIGUEZ, ENRIQUE M

JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2016 vol. 186 p. 181 - 191

0174-1578

This study was aimed at determining the role ofthe crustacean hyperglycemic hormone (CHH) in the physiological compensation toboth saline and thermal stress, in the freshwater crayfish Cheraxquadricarinatus. By determining the expression of the CHH gene in the eyestalkof juvenile crayfish, we found that maximal induction of CHH was induced athigh salinity (10 g/L) and low temperature (20 °C). In order to investigate therole of CHH in the physiological compensation to such stressful conditions,recombinant CHH was supplied to stressed animals. CHHinjected crayfish showedincreased hemolymphatic levels of glucose, in accordance with a significantutilization of glycogen reserves from the hepatopancreas. Furthermore, CHHadministration allowed stressed animals to regulate hemolymphatic sodium andpotassium at more constant levels than controls. Taken together, these resultssuggest a relevant role of CHH in increasing the energy available intended forprocesses involved in the physiological compensation of C. quadricarinatusto both saline and thermal  stress.