INVESTIGADORES
GOLDSTEIN RAIJ jorge
artículos
Título:
Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide.
Autor/es:
GOLDSTEIN J, SILBERSTEIN C, IBARRA C.
Revista:
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
Editorial:
Associacao Brasileira De Divulgacao Cientifica
Referencias:
Año: 2002 vol. 35 p. 145 - 151
ISSN:
0100-879X
Resumen:
Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3',5'-cyclic
AMP from ATP. These isoforms are critically involved in the regulation
of gene transcription, metabolism, and ion channel activity among
others. Nitric oxide (NO) is a gaseous product whose synthesis from
L-arginine is catalyzed by the enzyme NO synthase. It has been well
established that NO activates the enzyme guanylyl cyclase, but little
has been reported on the effects of NO on other important second
messengers, such as AC. In the present study, the effects of sodium
nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells
transfected with plasmids containing AC types I, II, V and VI were
evaluated. Total inhibition (approximately 98.5%) of cAMP production
was observed in COS-7 cells transfected with the AC I isoform and
previously treated with SNP (10 mM) for 30 min, when stimulated with
ionomycin. A high inhibition (approximately 76%) of cAMP production was
also observed in COS-7 cells transfected with the AC VI isoform and
previously treated with SNP (10 mM) for 30 min, when stimulated with
forskolin. No effect on cAMP production was observed in cells
transfected with AC isoforms II and V.