Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials

A. NOEL GRAVINA; KARIM MAGHNI; MÉLANIE WELMAN; L'HOCINE YAHIA; DORIS A. MBEH; PAULA V. MESSINA

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 1860 p. 452 - 464

0304-4165

Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovative therapeutic tools due to their exceptional antioxidants effects; nevertheless their bio-applications are still in their infancy. Methods: TiO2, Ce-TiO2 and CeO2-TiO2 NMs were synthesized by a bottom-up microemulsion- mediated strategy and compared to elucidate the physicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balance between the Ce3+/ Ce4+ on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts were selected as cell model because of their participation in wound healing and fibrotic diseases. Results: Ce-TiO2 NM obtained via sol-gel reaction chemistry of metallic organic precursors exerts a real cytoprotective effect against H2O2 over fibroblast proliferation, while CeO2 pre-formed nanoparticles incorporated to TiO2 crystalline matrix lead to a harmful CeO2-TiO2 material. TiO2 was processed by the same pathways as Ce-TiO2 and CeO2-TiO2 NM but did not elicit any adverse or protective influence compared to controls.Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material?s physicochemical properties and its subsequent influence in the cellular response. It can induce a range of biological reactions that vary from cytotoxic to cytoprotective.General Significance: Even though there are still some unresolved issues and challenges, the unique physical and chemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.