INVESTIGADORES
BUSTOS Diego Martin
artículos
Título:
Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress
Autor/es:
DIEGO M. BUSTOS; CLAUDIA A. BUSTAMANTE; ALBERTO A. IGLESIAS
Revista:
JOURNAL OF PLANT PHYSIOLOGY
Editorial:
Elsevier
Referencias:
Año: 2008 vol. 165 p. 456 - 461
ISSN:
0176-1617
Resumen:
Glyceraldehyde-3-phosphate dehydrogenases catalyze key steps in energy and
reducing power partitioning in cells of higher plants. Because non-phosphorylating
glyceraldehyde-3-phosphate dehydrogenase (NP-Ga3PDHase) is involved in the
production of reductive power (NADPH) in the cytosol, its behavior under oxidative
stress conditions was analyzed. The specific activity of the enzyme was found to
increase up to 2-fold after oxidative conditions imposed by methylviologen in wheat
and maize seedlings. Under moderate oxidant concentration, lack of mRNA induction
was observed. The increase in specific activity would thus be a consequence of a
significant stability of NP-Ga3PDHase. Our results suggest that the enzyme could be
modified by oxidation of cysteine residues, but formation of disulfide bridges is
dependent on levels of divalent cations and 14-3-3 proteins. The latter differential
effect could be critical to relatively maintain energy and reductant levels in the
cytoplasm of plant cells under oxidative stress.