THE FRACTAL SLOPE: A NONLINEAR APPROACH TO STUDY THE DESHIALIZATION CAUSED BY MUSCULAR LARVAE OF TRICHINELLA SPIRALIS

LEGUTO ALCIDES; KOROL ANA; BORTOLATO S.A.; PONCE DE LEÓN P.; LUPO MAELA

BIOCELL

Biocell

Lugar: Mendoza; Año: 2014 vol. 38

The Trichinella spiralis parasite, is a pathogen that caused the triquinosis, which infected form is the cyst of tricchina. The cellular mechanisms that appears during this process are not well known, but it was communicated that on one hand would participate the glucoconjugated from the parasite and the host and on the other previous experiences had shown that the muscular larvae captured the sialic acid, this suggest the possibility that the helmint could take it from the muscular cells. In this work we studied the erythrocytes aggregation produced by the in vitro contact between red blood cells (GR) and muscular larvae, through the Fractal Slope (D), using digital image processing, with free software FRACTALYSE. To obtain D we followed an iterative process, making a zoom on the image details. We analysed mathematically step level vs. the number of occupied spaces. On answering the main object of this work, we used GR type O as model cells, which were incubated in equal parts with the larvae concentrated. We work with 6 GR control samples, and larvae concentrations at 4500-5000 larvae/mL y 8500-9000 larvae/mL, at time in minutes: T1=0,T2=60, T3=120, in 4 experiences, obtained images by duplicated. The data obtained was normalized and no normalized, choosing the first one that offers better discrimination power. The fractal slope results: a) constant for control GR: D0=1.49±0.19, D60=1.44±0.22, D120=1.49±0.24, While for GRlarvae at T2, appears an increase of 10% and T3 a decreased of 7%. This would show the larvae interaction with GR, produced a change on the fractal patterns which appears as a change on D. These preliminary results will be completed with new experiences at intermediate times, to provide consistency to the results.