INVESTIGADORES
SEIJO jose guillermo
artículos
Título:
FIDELa retrovirus-like retrotransposon and its distinct evolutionary histories in the A- and B-genome components of cultivated peanut
Autor/es:
NIELEN S.; FERNANDO CAMPOS-FONSECA; SORAYA LEAL-BERTIOLI; P.M. GUIMARÃES,; SEIJO, JG; CHRISTOPHER TOWN; ROBERTO ARRIAL; BERTIOLI, D.J
Revista:
CHROMOSOME RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2010 vol. 18 p. 227 - 246
ISSN:
0967-3849
Resumen:
Abstract In this paper, we describe a Ty3-gypsy
retrotransposon from allotetraploid peanut (Arachis
hypogaea) and its putative diploid ancestors Arachis
duranensis (A-genome) and Arachis ipaënsis (Bgenome).
The consensus sequence is 11,223 bp. The
element, named FIDEL (Fairly long Inter-Dispersed
Euchromatic LTR retrotransposon), is more frequent
in the A- than in the B-genome, with copy numbers
of about 3,000 (±950, A. duranensis), 820 (±480, A.
ipaënsis), and 3,900 (±1,500, A. hypogaea) per
haploid genome. Phylogenetic analysis of reverse
transcriptase sequences showed distinct evolution of
FIDEL in the ancestor species. Fluorescent in situ
hybridization revealed disperse distribution in euchromatin
and absence from centromeres, telomeric
regions, and the nucleolar organizer region. Using
paired sequences from bacterial artificial chromosomes,
we showed that elements appear less likely to insert
near conserved ancestral genes than near the fast
evolving disease resistance gene homologs. Within the
Ty3-gypsy elements, FIDEL is most closely related
with the Athila/Calypso group of retrovirus-like retrotransposons.
Putative transmembrane domains were
identified, supporting the presence of a vestigial
envelope gene. The results emphasize the importance
of FIDEL in the evolution and divergence of different
Arachis genomes and also may serve as an example of
the role of retrotransposons in the evolution of legume
genomes in general.