INVESTIGADORES
BRACAMONTE angel guillermo
artículos
Título:
Supramolecular analytical chemistry: spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropy-beta-cyclodextrin
Autor/es:
A. GUILLERMO BRACAMONTE, GUADALUPE G. MIñAMBRES AND ALICIA V. VEGLIA
Revista:
ARKIVOC - ONLINE JOURNAL OF ORGANIC CHEMISTRY
Editorial:
ARKAT USA INC
Referencias:
Año: 2011 vol. vii p. 439 - 449
ISSN:
1551-7004
Resumen:
The effect of the addition of native (á, â and ã) and derivative (methyl-â and hydroxypropyl-â)
cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was
studied. The fluorescence of the substrate in the presence of hydroxypropyl-â-CD (HPCD)
showed the maximum enhancement and the association constant (KA, mol-1 L) was determined
(60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free
substrate (ö6HMCD/ö6HM) = 6.0±0.2 provides analytical advantages. On the basis of this
supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the
determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of
the method was performed in urine samples with very good recoveries 95-109 %.á, â and ã) and derivative (methyl-â and hydroxypropyl-â)
cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was
studied. The fluorescence of the substrate in the presence of hydroxypropyl-â-CD (HPCD)
showed the maximum enhancement and the association constant (KA, mol-1 L) was determined
(60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free
substrate (ö6HMCD/ö6HM) = 6.0±0.2 provides analytical advantages. On the basis of this
supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the
determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of
the method was performed in urine samples with very good recoveries 95-109 %.â-CD (HPCD)
showed the maximum enhancement and the association constant (KA, mol-1 L) was determined
(60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free
substrate (ö6HMCD/ö6HM) = 6.0±0.2 provides analytical advantages. On the basis of this
supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the
determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of
the method was performed in urine samples with very good recoveries 95-109 %.A, mol-1 L) was determined
(60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free
substrate (ö6HMCD/ö6HM) = 6.0±0.2 provides analytical advantages. On the basis of this
supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the
determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of
the method was performed in urine samples with very good recoveries 95-109 %.ö6HMCD/ö6HM) = 6.0±0.2 provides analytical advantages. On the basis of this
supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the
determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of
the method was performed in urine samples with very good recoveries 95-109 %.-1. The validation of
the method was performed in urine samples with very good recoveries 95-109 %.
