Participation of transcription factors from the Rel/NF-kappaB family in the circadian system.

LUCIANO MARPEGÁN,; TRISTAN BEKINSCHTEIN,; RAMIRO FREUDENTHAL,; MARÍA RUBIO,; GABRIELA FERREYRA,; ARTURO GABRIEL ROMANO; DIEGO GOLOMBEK,

NEUROSCIENCE LETTERS

Año: 2004 vol. 358 p. 9 - 12

0304-3940

We have studied the presence and activity of components of the nuclear factor-kB (NF-kB) transcription factor in the hamster circadian system analyzing wheel-running activity, protein expression and DNA binding activity by electrophoresis mobility shift assays (EMSA). Non-rhythmic specific immunoreactive bands corresponding to a NF-kB subunit (p65) were found in hamster suprachiasmatic nuclei (SCN) homogenates. The active form of NF-kB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NFkB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle þ light pulse: 2.08 ^ 0.46 h, PDTC þ light: 0.36 ^ 0.35 h). These results demonstrate the presence and activity of Rel/NF-kB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.kB (NF-kB) transcription factor in the hamster circadian system analyzing wheel-running activity, protein expression and DNA binding activity by electrophoresis mobility shift assays (EMSA). Non-rhythmic specific immunoreactive bands corresponding to a NF-kB subunit (p65) were found in hamster suprachiasmatic nuclei (SCN) homogenates. The active form of NF-kB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NFkB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle þ light pulse: 2.08 ^ 0.46 h, PDTC þ light: 0.36 ^ 0.35 h). These results demonstrate the presence and activity of Rel/NF-kB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.kB subunit (p65) were found in hamster suprachiasmatic nuclei (SCN) homogenates. The active form of NF-kB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NFkB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle þ light pulse: 2.08 ^ 0.46 h, PDTC þ light: 0.36 ^ 0.35 h). These results demonstrate the presence and activity of Rel/NF-kB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.kB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NFkB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle þ light pulse: 2.08 ^ 0.46 h, PDTC þ light: 0.36 ^ 0.35 h). These results demonstrate the presence and activity of Rel/NF-kB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.þ light pulse: 2.08 ^ 0.46 h, PDTC þ light: 0.36 ^ 0.35 h). These results demonstrate the presence and activity of Rel/NF-kB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.^ 0.46 h, PDTC þ light: 0.36 ^ 0.35 h). These results demonstrate the presence and activity of Rel/NF-kB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.