INVESTIGADORES
HOLLMANN Axel
artículos
Título:
Isolation, characterization and technological properties
Autor/es:
BRAVO FERRADA, B; DELFEDERICO, L; HOLLMANN, A; VALEZ LA HENZ, D; CURYLEN, Y; CABALLERO, A; SEMORILE, L
Revista:
International journal of microbiology research
Editorial:
Bioinfo
Referencias:
Año: 2011 vol. 3 p. 48 - 52
ISSN:
0975-5276
Resumen:
Pinot noir vinifications carried out at industrial scale during 2008 vintage were monitored in Patagonian
region, Argentina, and several lactic acid bacteria were obtained. By analysis of a fragment of rpoB gene, speciesspecific
PCR of malolactic enzyme and sequencing of 16S rRNA gene, three isolates were identified as
Oenococcus oeni. Polyphasic typing made by carbohydrate fermentation behaviour and RAPD-PCR grouped the
isolates and O. oeni reference strain in three different clusters. Additionally, the effect of ethanol on bacterial
growth and malolactic activity was evaluated. In spite of some inhibitory effects were found, the three isolates were
able to growth in all ethanol concentration tested. A clear correlation was found between the different clusters
obtained and their ethanol tolerance. All indigenous O. oeni showed a strong malolactic activity at all ethanol
concentrations assayed, indicating that this alcohol just affect the growing parameters of bacteria, but not the
malolactic activity. These promissory results suggest a potential enological application of the O. oeni indigenous
isolates as malolactic fermentation starters in winemaking.