INVESTIGADORES
ROTSTEIN Nora Patricia
artículos
Título:
Oxidative stress enhances the proliferation and dedifferentiation of retina glial cells in vitro.
Autor/es:
ABRAHAN C.E.; INSUA MF; POLITI L.E.; GERMAN O.L.,; ROTSTEIN N.P.
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Editorial:
Wiley Interscience-Blackwell
Referencias:
Año: 2009 vol. 87 p. 964 - 977
ISSN:
0360-4012
Resumen:
<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0in; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES; mso-fareast-language:ES;} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.25in 1.0in 1.25in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> Oxidative damage is involved in triggering neuronal death in several retina neurodegenerative diseases. The recent finding of stem cells in the retina has suggested that both preventing neuronal death and replacing lost neurons might be useful strategies for treating these diseases. We have previously shown that oxidative stress induces apoptosis in cultured retinal neurons. We now investigated the response of Müller cells, proposed as retina stem cells, to this damage. Treatment of glial cell cultures prepared from rat retinas with the oxidant paraquat (PQ) did not induce glial cell apoptosis. Instead, PQ promoted their rapid dedifferentiation and proliferation. PQ decreased expression of a marker of differentiated glial cells, simultaneously increasing the expression of smooth muscle actin (SMA), shown to increase with glial dedifferentiation, the levels of cell cycle markers and the amount of glial cells in the cultures. In addition, glial cells protected neurons in co-culture from apoptosis induced by PQ and H2O2. In pure neuronal cultures, PQ induced apoptosis of photoreceptors and amacrine neurons, simultaneously decreasing the percentage of neurons preserving mitochondrial membrane potential; co-culturing neurons with glial cells completely prevented PQ-induced apoptosis and preserved mitochondrial potential in both neuronal types. These results demonstrate that oxidative damage activated different responses in Müller glial cells; they rapidly dedifferentiated and enhanced their proliferation, concurrently preventing neuronal apoptosis. Glial cells might not only preserve neuronal survival but also activate their cell cycle to provide a pool of new progenitor cells that might eventually be manipulated to preserve retina functionality.