Bradykinin stimulation of inositol phosphate and calcium responses in insensitive to pertussis toxin in NG115-401L neuronal cells

TREVOR R. JACKSON; SEAN I. PATTERSON; YUNG H. WONG; MICHAEL R. HANLEY

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Año: 1987 vol. 148 p. 412 - 416

0006-291X

Stimulation of NG115-401L neuronal cells with bradykinin produces a dose-dependent increase in inositol phosphate production which is not blocked, rather slightly increased, after treatment with pertussis toxin. Nevertheless, pertussis toxin stimulates ADP-ribosylation of a 41K membrane protein, and blocks opioid receptor-mediated inhibition of stimulated cAMP production in these cells. These results suggest that bradykinin responses in the NG115-401L cells are pertussis-insensitive, unlike bradykinin responses reported in other neuronal cell lines.