Rapid determination of retinoic acid and its main isomers in plasma by second order high-performance liquid chromatography data modeling

TEGLIA C; CAMARA, MS; GOICOECHEA H C

ANALYTICAL AND BIOANALYTICAL CHEMISTRY

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2014 vol. 406 p. 7989 - 7998

1618-2642

This work presents the development of a method based on high performance liquid chromatography (HPLC) coupled to second-order data modeling with multivariate curve resolution-alternating least-squares (MCR?ALS) for the quantitation of retinoic acid and its main isomers in plasma in only 5.5 min. The compounds retinoic acid, 13?cis?retinoic acid, 9?cis?retinoic acid and 9,13?di?cis?retinoic acid were partially separated using a Poroshell 120 EC?C18 (3.0 x 30 mm, 2.7 µm particle size) column. The overlapping not only among the target analytes but also with the plasma interferents was solved by exploiting the second-order advantage of the multi-way calibration. A validation study was performed obtaining the following results: trueness with recoveries of 98.5?105.9 % for RA, 95.7?110.1 % for 13?cis?RA, 97.1?110.8 % for 9?cis?RA and 99.5?110.9 % for 9,13?di?cis?RA; repeatability with RSD% of 3.5?3.1 % for RA, 3.5?1.5 % for 13?cis?RA, 4.6?2.7 % for 9?cis?RA and 5.2?2.7 % for 9,13?di?cis?RA (low and high levels); and intermediate precision (inter-day precision) with RSD% of 3.8?3.0 % for RA, 2.9?2.4 % for 13?cis?RA, 3.6?3.2 % for 9,13?di?cis?RA and 3.2?2.9 % for 9?cis?RA (low and high levels). In addition, a robustness study allowed to conclude about the method applicability to control patients undertaken the pharmaceutical products containing RA and 13?cis?RA in the treatment of dermatological diseases.