Separation and quantitation of enantiomeric 3,6-anhydrogalactoses by conversion to the corresponding diastereomeric acetylated sec-butyl 3,6-anhydrogalactonates

MARÍA ERREA; MARINA CIANCIA; MARÍA MATULEWICZ; ALBERTO CEREZO

CARBOHYDRATE RESEARCH

Elsevier Science Ltd.

Lugar: Oxford, Gran Bretaña; Año: 1998 vol. 311 p. 235 - 238

0008-6215

A method to determine the proportion of both enantiomers of 3,6-anhydrogalactose in a sample has been developed using methyl 3,6-anhydro-a-D-galactoside as a model compound. It involves an oxidative hydrolysis to obtain the corresponding aldonic acids, which are further converted to the acetylated diastereomeric sec-butyl esters. These are separated and quantified by GLC using a standard column for carbohydrate analysis; the procedure has been extended to seaweed polysaccharides.