Application of Pressure Probe and UV-MALDI TOF MS for direct Analysis of Plant Underivatized Carbohydrates in Sub-picoliter Single-Cell Cytoplasm Extract

,YOUSEF GHOLIPOUR; HIROSHI NONAMI; ROSA ERRA-BALSELLS,

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Elsevier

Lugar: Amsterdam; Año: 2008 vol. 19 p. 1841 - 1848

1044-0305

Application of Pressure Probe and UV-MALDI-TOF MS for direct Analysis of Plant Underivatized Carbohydrates in Sub-picoliter Single-Cell Cytoplasm Extract Yousef Gholipour The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan. Hiroshi Nonami  Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan. Rosa Erra-Balsells[1] CIHIDECAR-CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabell¨®n II, 3 P, Ciudad Universitaria, 1428-Buenos Aires, Argentina. Running title: UV-MALDI-MS of plant single-cell extract Abstract Single-cell cytoplasm sap (1-10 pL) was extracted by using Pressure Probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrates content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP) and carbon nanotubes (CNTs) in positive ion mode were selected for analysis because of acceptable carbohydrate-related signal reproducibility. Disaccharide and oligosaccharide (up to 15Hex when THAP was used, 11Hex with DHB and 7Hex with CNTs) were detected in tulip bulb cell cytoplasm sample. By using DHB as matrix, neutral carbohydrates were more abundantly detected as sodiated cations; the sugar-related signals however appeared as dominant potassiated cations when using THAP and CNTs. Small amount of monosaccharide was also detected in bulb cell cytoplasm with CNTs as matrix. UV-MALDI-TOF MS of leaf cell extract resulted in high-resolution detection of hexose and disaccharide with DHB, THAP and CNTs. [1] corresponding author; Address reprint requests to Rosa Erra-Balsells, CIHIDECAR-CONICET, Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabell¨®n II, 3 P, Ciudad Universitaria, 1428-Buenos Aires, Argentina. Tel/Fax: +54-11-45763346 erra@qo.fcen.uba.ar¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡