INVESTIGADORES
GAMARRA LUQUES Carlos Diego
artículos
Título:
Uric acid accumulation within intracellular crystalloid corpuscles of the midgut gland in Pomacea canaliculata (Caenogastropoda, Ampullariidae).
Autor/es:
ISRAEL A. VEGA, MAXIMILIANO GIRAUD-BILLOUD, EDUARDO KOCH, CARLOS GAMARRA-LUQUES & ALFREDO CASTRO-VAZQUEZ.
Revista:
VELIGER
Editorial:
Allen Press
Referencias:
Lugar: California; Año: 2007 vol. 48 p. 276 - 283
ISSN:
0042-3211
Resumen:
ABSTRACT
Pomacea canaliculata shows sleeves of a specialized tissue surrounding arteries
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.
shows sleeves of a specialized tissue surrounding arteries
of the midgut gland. This tissue is formed mainly by radiated cells (large
vacuolated cells, bearing small nuclei without nucleoli) which are arranged
around the vascular muscular layer. Smaller satellite cells, with a scarce
cytoplasm and clear nucleolated nuclei, are found together with some nerve
endings and muscle fibers on the external surface of these sleeves. Radiated
cells are full of crystalloid spheres (about 5 ìm diameter) showing a complex
inner fibrillar structure. Crystalloid corpuscles, and pigmented C and K corpuscles
present in midgut gland alveoli, were isolated from gland homogenates. Uric acid,
urea, ammonia, protein and calcium were determined in both gland homogenates
and corpuscular fractions. Uric acid was the major non-protein nitrogen
compound of the midgut gland and was concentrated in crystalloid corpuscles
(accounting for 53% of corpuscular dry mass), but was not detected in pigmented
corpuscles. Calcium accounted for only 0.6% of crystalloid dry mass. Protein was
another significant component of crystalloid bodies (32% of dry mass). Ammonia,
followed by urea, was the main nitrogen excretory product in the soluble fraction
of excreta, while protein predominated in the particulate excretory fraction. The
physiological significance of uric acid storage in such specialized tissue is
discussed.