Biochemical characterization of metallo-beta-lactamase VIM-11 from a Pseudomonas aeruginosa clinical strain.

MARCHIARO P; TOMATIS PE; MUSSI MA; PASTERAN F; VIALE AM; LIMANSKY AS; VILA AJ

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Año: 2008 p. 2250 - 2252

0066-4804

A detailed biochemical characterization of the Pseudomonas aeruginosa VIM-11 metallo-beta-lactamase (MbetaL) is reported. The only substitution differentiating VIM-11 from VIM-2 (N165S) promoted a slightly improved catalytic efficiency of the former on 3 out of 12 substrates, notably the bulky cephalosporins. Thus, MbetaL-mediated resistance also may be modulated by remote mutations.