INVESTIGADORES
BALZARINI Monica Graciela
artículos
Título:
Microsatélites markers linked to QTL for resistance to Mal de Río Cuarto disease in Zea mays L.
Autor/es:
DI RENZO M; BONAMICO N; DIAZ D; IBAÑEZ M; BALZARINI M; SALERNO J
Revista:
JOURNAL OF AGRICULTURAL SCIENCE
Editorial:
Cambridge University Press
Referencias:
Año: 2004 vol. 142 p. 289 - 295
ISSN:
0021-8596
Resumen:
Mal de Rý´o Cuarto (MRC) disease, caused by a member of the family Reoviridae belonging to the
genus Fijivirus, is considered to be the most damaging viral disease of maize (Zea mays L.) in
Argentina. Resistance to MRC disease is a quantitative trait with moderate heritability ranging from
0.44 to 0.56. The objective of this study was to identify SSR loci linked to quantitative trait loci (QTL)
contributing to MRC disease resistance. Two hundred and twenty-seven F3 derived-lines from a cross
between a susceptible inbred line, Mo17, and a partially resistant inbred line, BLS14, were evaluated
across four Rý´o Cuarto environments. A disease severity index (DSI) based on disease grades was
calculated and used to rate F3 derived-lines for their resistance to MRC disease. A subset of parental
F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed
for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing
of linkage among DNA markers and the mapping of QTL through composite interval mapping.
Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance
and dominant gene action, respectively. A simultaneous fit with these QTL in the joint
analyses explained 36.2% of the phenotypic variance. In spite of the fact that relative efficiency of
marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped
QTL could improve the efficiency of efforts in breeding for resistance to MRC diseaseReoviridae belonging to the
genus Fijivirus, is considered to be the most damaging viral disease of maize (Zea mays L.) in
Argentina. Resistance to MRC disease is a quantitative trait with moderate heritability ranging from
0.44 to 0.56. The objective of this study was to identify SSR loci linked to quantitative trait loci (QTL)
contributing to MRC disease resistance. Two hundred and twenty-seven F3 derived-lines from a cross
between a susceptible inbred line, Mo17, and a partially resistant inbred line, BLS14, were evaluated
across four Rý´o Cuarto environments. A disease severity index (DSI) based on disease grades was
calculated and used to rate F3 derived-lines for their resistance to MRC disease. A subset of parental
F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed
for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing
of linkage among DNA markers and the mapping of QTL through composite interval mapping.
Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance
and dominant gene action, respectively. A simultaneous fit with these QTL in the joint
analyses explained 36.2% of the phenotypic variance. In spite of the fact that relative efficiency of
marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped
QTL could improve the efficiency of efforts in breeding for resistance to MRC diseaseFijivirus, is considered to be the most damaging viral disease of maize (Zea mays L.) in
Argentina. Resistance to MRC disease is a quantitative trait with moderate heritability ranging from
0.44 to 0.56. The objective of this study was to identify SSR loci linked to quantitative trait loci (QTL)
contributing to MRC disease resistance. Two hundred and twenty-seven F3 derived-lines from a cross
between a susceptible inbred line, Mo17, and a partially resistant inbred line, BLS14, were evaluated
across four Rý´o Cuarto environments. A disease severity index (DSI) based on disease grades was
calculated and used to rate F3 derived-lines for their resistance to MRC disease. A subset of parental
F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed
for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing
of linkage among DNA markers and the mapping of QTL through composite interval mapping.
Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance
and dominant gene action, respectively. A simultaneous fit with these QTL in the joint
analyses explained 36.2% of the phenotypic variance. In spite of the fact that relative efficiency of
marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped
QTL could improve the efficiency of efforts in breeding for resistance to MRC disease3 derived-lines from a cross
between a susceptible inbred line, Mo17, and a partially resistant inbred line, BLS14, were evaluated
across four Rý´o Cuarto environments. A disease severity index (DSI) based on disease grades was
calculated and used to rate F3 derived-lines for their resistance to MRC disease. A subset of parental
F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed
for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing
of linkage among DNA markers and the mapping of QTL through composite interval mapping.
Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance
and dominant gene action, respectively. A simultaneous fit with these QTL in the joint
analyses explained 36.2% of the phenotypic variance. In spite of the fact that relative efficiency of
marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped
QTL could improve the efficiency of efforts in breeding for resistance to MRC disease3 derived-lines for their resistance to MRC disease. A subset of parental
F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed
for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing
of linkage among DNA markers and the mapping of QTL through composite interval mapping.
Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance
and dominant gene action, respectively. A simultaneous fit with these QTL in the joint
analyses explained 36.2% of the phenotypic variance. In spite of the fact that relative efficiency of
marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped
QTL could improve the efficiency of efforts in breeding for resistance to MRC disease2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed
for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing
of linkage among DNA markers and the mapping of QTL through composite interval mapping.
Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance
and dominant gene action, respectively. A simultaneous fit with these QTL in the joint
analyses explained 36.2% of the phenotypic variance. In spite of the fact that relative efficiency of
marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped
QTL could improve the efficiency of efforts in breeding for resistance to MRC disease
