Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri) by Electron Paramagnetic Spin Resonance Spectrometry

DÍAZ, ANA CRISTINA; FERNÁNDEZ GIMENEZ, ANALIA VERÓNICA; MENDIARA, SARA NOEMÍ; FENUCCI JORGE LINO

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

American Chemical Society

Lugar: Published on Web; Año: 2004 vol. 52 p. 3189 - 3193

0021-8561

Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri) by Electron Paramagnetic Spin Resonance Spectrometry Spectrometry Spectrometry Spectrometry Spectrometry muelleri) by Electron Paramagnetic Spin Resonance Spectrometry Spectrometry Spectrometry Spectrometry Spectrometry muelleri) by Electron Paramagnetic Spin Resonance Spectrometry Spectrometry Spectrometry Spectrometry Spectrometry muelleri) by Electron Paramagnetic Spin Resonance Spectrometry Spectrometry Spectrometry Spectrometry Spectrometry muelleri) by Electron Paramagnetic Spin Resonance Spectrometry Spectrometry Spectrometry Spectrometry Spectrometry Pleoticus muelleri) by Electron Paramagnetic Spin Resonance Spectrometry Spectrometry Spectrometry Spectrometry Spectrometry ) by Electron Paramagnetic Spin Resonance Spectrometry ANA C. DIÄAZ,*,† ANALIÄA V. FERNAÄ NDEZ GIMENEZ,§ SARA N. MENDIARA, ANDNA C. DIÄAZ,*,† ANALIÄA V. FERNAÄ NDEZ GIMENEZ,§ SARA N. MENDIARA, AND JORGE L. FENUCCI§ORGE L. FENUCCI§ Departamento Ciencias Marinas, Universidad Nacional de Mar del Plata, Funes 3350, 7600 Mar del Plata, Argentina Free radical scavenging properties of hepatopancreas extracts of Pleoticus muelleriwere evaluated by electron paramagnetic spin resonance spectrometry methods (EPR) against the stable 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical. The present study was carried out to characterize different physiological stages of the shrimp under environmental and nutritional stress, evaluating the effect on growth, survival, and functional morphology of the hepatopancreas. Feeding trials were carried out on juveniles (1 g initial weight) held in aquaria. Each diet, with different concentrations of vitamins A and E, was tested in triplicate groups during 25 days. The control groups were fed with fresh squid mantle and with a vitamin-free diet. For all of the diets, the extracts exhibited strong DPPH radical scavenging activity, suggesting that the tissue is a powerful natural antioxidant. Individuals fed with different concentrations of vitamin E showed the strongest effect on the DPPH radicals, reducing the DPPH radicals to 50%, after an incubation period of 3 min. In contrast, the extracts of control animals, fed with squid mantle, had the weakest antioxidant activity (4%). These data indicated that the presence of vitamin E in the diet can provide immediate protection against free radicals. KEYWORDS: Free radical scavenging; DPPH; electron paramagnetic spin resonance; antioxidant activity; hepatopancreas; Pleoticus muelleri