INVESTIGADORES
ROZENFELD paula Adriana
artículos
Título:
Detection and identification of a soy protein component that cross react with caseins from cow milk
Autor/es:
ROZENFELD PA; DOCENA GH; ANON MC; FOSSATI CA
Revista:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Referencias:
Año: 2002 vol. 130 p. 49 - 58
ISSN:
0009-9104
Resumen:
Soy-based formulas are the most employed cows milk substitutes in the treatment of cows milk allergy
in our country. Since adverse reactions have been reported in allergic patients as a consequence of
exposure to soy proteins, we have investigated the possible cross-reactivity between components from
soybean and cows milk. A cows milk specific polyclonal antiserum and casein specific monoclonal antibodies
were used in immunoblotting and competitive ELISA studies to identify a 30-kD component
from soybean that cross-reacts with cows milk caseins. Its IgE binding capacity was tested by EAST,
employing sera from cows milk allergic patients, not previously exposed to soy proteins. The 30kD
protein was isolated and partially sequenced. It is constituted by two polypeptides (A5 and B3) linked
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B3
5 and B3) linked
by a disulphide bond. The proteins capacity to bind to the different antibodies relies on the B33
poly-peptide. These results indicate that soy-based formula, which contains the A5-B3 glycinin
molecule, could be involved in allergic reactions observed in cows milk allergic patients exposed to
soy-containing foods.