Spatiotemporal expression of Rhomboid domain containing 2(Rhbdd2) during rat development

FERRETI VA; CANZONERI R; BARBEITO CG.; CROCE MV; ABBA MC; LACUNZA E

ACTA HISTOCHEMICA

ELSEVIER GMBH, URBAN & FISCHER VERLAG

Lugar: ALEMANIA; Año: 2015

0065-1281

tOver the last few years rhomboid genes have gained interest because of its association with cancerand neurodegenerative diseases. In previous studies, we demonstrated that human RHBDD2 is over-expressed in the advanced stages of breast and colorectal cancers, suggesting a favorable role in cellproliferation. So far little is known about the expression of RHBDD2 in other tissues and other species,and because of similarities between cancer and embryonic cells, this study focused on the evaluation ofRhbdd2 expression in embryonic and adult rat tissues. By IHC and RT-PCR, Rhbdd2 was identified in earlystages of most tissues analyzed, with high expression in brain, spinal cord, kidney and embryonic skin.In adult tissues, the expression remained elevated while salivary glands became positive. Furthermore,Rhbdd2 showed a high expression in the most proliferative stages of the rat mammary gland. Indeed,similar findings were observed in the mouse mammary epithelial cell line HC11, in which Rhbdd2 residesin the Golgi apparatus, and at different stages of mouse mammary gland development. Therefore, Rhbdd2would be implicated in embryonic and adult tissue proliferation.