Matrix-assisted ultraviolet laser desorption/ionization time-of-flight (UV-MALDI-TOF) mass spectra of N-acylated and N,O-acylated glycosylamines

YASUTO SATO; YUKO FUKUYAMA; HIROSHI NONAMI; ROSA ERRA-BALSELS; CARLOS A. STORTZ; ALBERTO S. CEREZO; MARÍA C. MATULEWICZ

CARBOHYDRATE RESEARCH

Elsevier

Lugar: Amsterdam; Año: 2007 vol. 342 p. 2567 - 2574

0008-6215

Matrix-assisted ultraviolet laser desorption/ionization time-of-.ight mass spectrometry (UV-MALDI-TOF-MS) has shown to be a very useful technique for the study of the non-volatile and thermally non-stable N-acylated glycopyranosyl- and glycofuranosyl-amines. Of the several matrices tested, 2,5-dihydroxybenzoic acid (DHB) was the most e.ective giving good spectra in the positive-ion mode. In the linear and re.ectron modes, the [M+Na]+ ions appeared with high intensity. Their fragmentation patterns were investigated by post-source decay (PSD) UV-MALDI-TOF-MS showing mainly cross-ring cleavages. In addition, N,O-acylated glycopyranosyl- and glycofuranosyl-amines were also analyzed by this technique. PSD UV-MALDITOF-MS gave signi.cant signals for several primary fragment ions, which were proposed but not detected, or observed with very low abundance, in electron ionization mass spectrometry (EI-MS) experiments.+ ions appeared with high intensity. Their fragmentation patterns were investigated by post-source decay (PSD) UV-MALDI-TOF-MS showing mainly cross-ring cleavages. In addition, N,O-acylated glycopyranosyl- and glycofuranosyl-amines were also analyzed by this technique. PSD UV-MALDITOF-MS gave signi.cant signals for several primary fragment ions, which were proposed but not detected, or observed with very low abundance, in electron ionization mass spectrometry (EI-MS) experiments.