INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Chemometrics-assisted simple UV-spectroscopic determination of carbamazepine in human serum and comparison with reference methods
Autor/es:
MS CÁMARA, C MASTANDREA AND HC GOICOECHEA*
Revista:
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2005 vol. 64 p. 153 - 166
ISSN:
0165-022X
Resumen:
In the present report, carbamazepine is determined on serum samples of real patients by a procedure completely assisted by chemometric tools. First, a response surface methodology based on a mixture design was applied in order to select the best conditions for the extraction step. Finally, partial least squares multivariate calibration (PLS-1) was applied to second derivative UV spectra, eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) a method comparison with high performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA) applied on real patient samples. The obtained results show the potentiality of the presently studied methodology for the monitoring of patients treated with this anticonvulsivant.
