INVESTIGADORES
ANZULOVICH MIRANDA Ana Cecilia
artículos
Título:
Daily rhythms of catalase and glutathione peroxidase expression and activity are endogenously driven in the hippocampus and are modified by a vitamin a-free diet
Autor/es:
NAVIGATORE FONZO LS; DELGADO SM; GIMÉNEZ MS; ANZULOVICH AC
Revista:
NUTRITIONAL NEUROSCIENCE
Editorial:
MANEY PUBLISHING
Referencias:
Lugar: London; Año: 2014 vol. 17 p. 21 - 30
ISSN:
1028-415X
Resumen:
Objectives. Alterations in enzymatic antioxidant defense systems lead to a deficit of cognitive functions and altered hippocampal synaptic plasticity. The objectives of this study were to investigate endogenous rhythms of catalase (CAT) and glutathione peroxidase (GPx) expression and activity, as well as CREB1 mRNA, in the rat hippocampus, and to evaluate to which extent the vitamin A deficiency could affect those temporal patterns. Methods. Rats from control and vitamin A-deficient groups received a diet containing 4000 IU of vitamin A/Kg diet, or the same diet devoided of vitamin A, respectively, during 3 months. Rats were maintained under 12 h-dark:12 h-dark conditions, during 10 days before the sacrifice. Circadian rhythms of CAT, GPx, RXRγ and CREB1 mRNA levels were determined by RT-PCR in hippocampus samples isolated every 4 h during a 24 h period. CAT and GPx enzymatic activities were also determined by kinetic assays. Regulatory regions of clock and antioxidant enzymes genes were scanned for E-box, RXRE and CRE sites. Results. E-box, RXRE and CRE sites were found on regulatory regions of GPx and CAT genes, which display a circadian expression in the rat hippocampus. Vitamin A deficiency (VAD) phase shifted CAT, GPx and RXRγ endogenous rhythms without affecting circadian expression of CREB1. Discussion. CAT and GPx expression and enzymatic activity are circadian in the rat hippocampus. The VAD affected the temporal patterns antioxidant genes expression, probably by altering circadian rhythms of its RXR receptors and clock factors; thus it would impair the temporal orchestration of hippocampal daily cognitive performance.