Immunological properties of FMDV-gP64 fusion proteins displayed on SF9 cell and baculovirus surfaces.

TAMI, CECILIA; ANDREA PERALTA; ROSARIO BARBIERI; ANALIA BERINSTEIN; CARRILLO, ELISA; TABOGA, OSCAR

Vaccine

Año: 2004 vol. 23 p. 840 - 845

In the present report, we characterized the immune response and the protection conferred by recombinant baculoviruses or infected insect cells expressing the fusions gp64-P1 and gp64-site A FMDV antigens. Mice, vaccinated intraperitoneally with gp64-P1 immunogens, showed a low-antibody response and a variable degree of protection. However, when mice received recombinant baculoviruses or infected insect cells expressing the fusion protein gp64-site A, high-ELISA and seroneutralizing titers (SNT) against FMDV were elicited. All mice immunized with Sf9 cells expressing FMDV site A developed a protective immune response against challenge with virulent FMDV, indicating that the baculovirus display of foreign epitopes is a promising approach to biosynthetic vaccines.