INVESTIGADORES
FABRA Adriana Isidora
artículos
Título:
SIGNAL MOLECULES IN THE PEANUT-BRADYRHIZOBIA INTERACTION
Autor/es:
TAURIAN,T.; MORÓN,B.; SORIA-DÍAZ, M.E.; ANGELINI,J.; TEJERO-MATEO,P; GIL-SERRANO, A.; MEGÍAS,M.; FABRA,A.
Revista:
ARCHIVES OF MICROBIOLOGY
Referencias:
Año: 2008 vol. 189 p. 345 - 356
ISSN:
0302-8933
Resumen:
Abstract
Main nodulation signal
molecules in the peanut-bradyrhizobia interaction were examined. Flavonoids exuded
by Arachis hypogaea L. cultivar Tegua
were genistein, daidzein and chrysin, the latest being released in lower
quantities. Thin layer chromatography analysis from genistein-induced bacterial
cultures of three peanut bradyrhizobia resulted in an identical Nod factor
pattern, suggesting low variability in genes involved in the synthesis of these
molecules. Structural study of Nod factor by mass spectrometry and NMR analysis revealed that it shares a variety of
substituents with the broad-host-range Rhizobium
sp NGR234 and Bradyrhizobium spp.
Nodulation assays in legumes nodulated by these rhizobia demonstrated
differences between them and the three peanut bradyrhizobia. The three isolates
were classified as Bradyrhizobium sp.
Their fixation gene nifD and the
common nodulation genes nodD and nodA were also analyzed.