INVESTIGADORES
MBAYED Viviana Andrea
artículos
Título:
Characteristic in vitro evolution pattern of foot and mouth disease virus 81/Castellanos/Arg/87
Autor/es:
MBAYED, V.; SCHIAPPACASSI, M.; COROMINAS, A.; CAMPOS, R.
Revista:
VIRUS RESEARCH
Editorial:
Elsevier
Referencias:
Lugar: Atlanta; Año: 1997 vol. 48 p. 157 - 163
ISSN:
0168-1702
Resumen:
The in vitro evolution of Foot and mouth disease virus (FMDV) A:81:Castellanos:Arg:87 (A:Castellanos:87) wasstudied by partial biological and biochemical characterization of viral populations selected after 25 passages onsecondary fetal bovine kidney cell monolayers. These passages were performed in the presence or absence of immunepressure exerted in the form of antiviral polyclonal serum. While the viral populations passaged in the absence ofimmune pressure acquired characteristics such as antigenic heterogeneity, VP1 amino acid modification and plaquesize reduction, the populations selected after immune pressure also presented both neutralizing resistance andattenuation for suckling mice. The comparison with other previously studied FMDV strains suggests that FMDVA:Castellanos:87 adopts a differential response to immunological pressure and other selective forces. In addition, thesequencing analysis of viral selected populations shows a restriction in the number and type of amino acidreplacements tolerated by FMDV capsid proteins.
