On line detection of low naphthalene concentrations by a bioluminescent sensor

E VALMAN; F. BATTAGLINI; S. LEITE; B. VALMAN

PROCESS BIOCHEMISTRY

Elsevier

Año: 2004 vol. 39 p. 1217 - 1222

0032-9592

On-line tests with Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence assays. A mathematical model showed that 0.2 g/l is the best cell concentration to be used in the detection of low naphthalene concentrations, with the highest acceleration rate of bioluminescence sensitivity increase (0.46 nA l/(g min2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3
M) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16
M), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA.Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence assays. A mathematical model showed that 0.2 g/l is the best cell concentration to be used in the detection of low naphthalene concentrations, with the highest acceleration rate of bioluminescence sensitivity increase (0.46 nA l/(g min2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3
M) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16
M), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA.2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3
M) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16
M), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA.
M) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16
M), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA.
M), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA.