INVESTIGADORES
LUQUET Carlos Marcelo
artículos
Título:
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity.
Autor/es:
14. LUQUET C; POSTEL U; URCOLA M; HALPERIN J; MARQUES R; SIEBERS D.
Revista:
JOURNAL OF EXPERIMENTAL BIOLOGY
Editorial:
The Company of Biologists
Referencias:
Lugar: Cambridge; Año: 2002 vol. 205 p. 71 - 77
ISSN:
0022-0949
Resumen:
We studied the transepithelial potential difference
(TEPD) and 22Na flux across isolated perfused gills
(anterior pair 5 and posterior pairs 68) of the crab22Na flux across isolated perfused gills
(anterior pair 5 and posterior pairs 68) of the crab
Chasmagnathus granulatus acclimated to either hypo- or
hyper-osmotic conditions.
The gills of crabs acclimated to low salinity, perfused
and bathed with 10 saline solutions, produced the
following TEPDs (hemolymph side with respect to bath
side): 0.4±0.7, 10.2±1.6, 10.8±1.3 and 6.7±1.3 mV for
gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not
differ significantly. Reducing the saline concentration of
bath and perfusate from 30 to 20 or 10 increased
significantly the TEPDs of these gills. TEPDs of gill 6
(representative of posterior gills) were reduced by 69±5%
and 60±5% after perfusion with ouabain or BaCl2acclimated to either hypo- or
hyper-osmotic conditions.
The gills of crabs acclimated to low salinity, perfused
and bathed with 10 saline solutions, produced the
following TEPDs (hemolymph side with respect to bath
side): 0.4±0.7, 10.2±1.6, 10.8±1.3 and 6.7±1.3 mV for
gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not
differ significantly. Reducing the saline concentration of
bath and perfusate from 30 to 20 or 10 increased
significantly the TEPDs of these gills. TEPDs of gill 6
(representative of posterior gills) were reduced by 69±5%
and 60±5% after perfusion with ouabain or BaCl22
(5mmoll1 each), respectively. The same gill showed a net
ouabain-sensitive Na+ influx of 1150±290 mequiv g1 h1.
Gill 6 of crabs acclimated to high salinity produced
TEPDs of 1.5±0.1 and 1.3±0.09 mV after perfusion with
30 or 40 salines, respectively. Perfusion with ouabain
or BaCl2 reduced TEPDs by 76±7% and 86±4%,
respectively. A net ouabain-sensitive Na+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.2 reduced TEPDs by 76±7% and 86±4%,
respectively. A net ouabain-sensitive Na+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.1 each), respectively. The same gill showed a net
ouabain-sensitive Na+ influx of 1150±290 mequiv g1 h1.
Gill 6 of crabs acclimated to high salinity produced
TEPDs of 1.5±0.1 and 1.3±0.09 mV after perfusion with
30 or 40 salines, respectively. Perfusion with ouabain
or BaCl2 reduced TEPDs by 76±7% and 86±4%,
respectively. A net ouabain-sensitive Na+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.2 reduced TEPDs by 76±7% and 86±4%,
respectively. A net ouabain-sensitive Na+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.+ influx of 1150±290 mequiv g1 h1.
Gill 6 of crabs acclimated to high salinity produced
TEPDs of 1.5±0.1 and 1.3±0.09 mV after perfusion with
30 or 40 salines, respectively. Perfusion with ouabain
or BaCl2 reduced TEPDs by 76±7% and 86±4%,
respectively. A net ouabain-sensitive Na+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.2 reduced TEPDs by 76±7% and 86±4%,
respectively. A net ouabain-sensitive Na+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.+ efflux of
2282±337 mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.mequiv g1h1 was recorded in gill 6 perfused
with 38 saline.