INVESTIGADORES
RODRIGUEZ Horacio Adolfo
artículos
Título:
Estrogen and progesterone modulation of eosinophilic infiltration of the rat uterine cervix
Autor/es:
RAMOS JG; VARAYOUD JG; KASS L; RODRIGUEZ HA; MUÑOZ DE TORO MM; LUQUE EH
Revista:
STEROIDS
Referencias:
Año: 2000 vol. 65 p. 409 - 414
ISSN:
0039-128X
Resumen:
Ripening of the rat cervix involves widespread collagenolysis that
follows an eosinophilic leukocyte infiltration. The hormonal control of
these events is not well understood. The aims of this study were to
investigate the mechanism through which progesterone (P) and
17beta-estradiol (E(2)) modulate eosinophilic invasion and to determine
if this event is protein synthesis mediated. Cervical eosinophilic
invasion was measured in intact rats during the second half of
pregnancy and compared with values from ovariectomized (O)
pseudopregnant (PSP) rats treated with P and E(2) in doses that
mimicked the levels of pregnancy. Other O-PSP rats were treated with an
E(2) antagonist (tamoxifen) and the antiprogestin RU-486. To study the
role of protein synthesis in eosinophilic invasion of the cervix, rats
were treated with actinomycin-D (an inhibitor of mRNA synthesis), and
animals were sacrificed on D21 or D22 to evaluate eosinophilic
invasion. Rats treated with E(2) showed high levels of infiltration and
tamoxifen blocked this E(2) effect. On the other hand, P antagonized
the stimulatory effects of E(2) on eosinophilic invasion, however when
the P and E(2) treated rats were injected with RU-486 the inhibitory
effect of P was reversed. In intact pregnant rats a sharp rise in
eosinophilic infiltration was detected on D23, 20 h after the fall of
serum P. Finally, E(2) treated rats injected with actinomycin-D had no
invasion of eosinophils. In conclusion, the estrogen-triggered
eosinophil invasion is affected by the classic estrogen receptor
antagonist tamoxifen and by the mRNA synthesis blocker actinomycin-D
suggesting a genomic action of E(2). Furthermore, the estrogen effect
is blocked by P and this inhibition is reversed by RU-486.