INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
The identification of sandfly species, from an area of species , from an area of Argentina, with endemic leishmaniasis , by de PCR based analysis of the gene coding for 18S ribosomal RNA.
Autor/es:
BARROSO, P.A., MARCO, J. D., KATO, H., TARAMA, R., P. RUEDA, P., S. CAJAL, S.P., BASOMBRÍO, M.A., KORENAGA, M., TARANTO, N.J. AND HASHIGUCHI, Y.
Revista:
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY
Referencias:
Año: 2007 vol. 101 p. 247 - 253
ISSN:
0003-4983
Resumen:
The area around Río Blanco, in the Ora´n department in the north of the Argentinian province of Salta, is endemic
for American tegumentary leishmaniasis. In an attempt to facilitate the identification of the Lutzomyia species in
this area, sequences of the gene coding for the 18S ribosomal RNA (rRNA) of sandflies caught in a Shannon trap
were explored, by a combination of PCR and analysis of restriction-fragment-length polymorphism (RFLP). The
products from the PCR, which employed two primers developed specifically for this study (Lu.18S 1S and Lu.18S
AR), were cloned into a commercial vector (pGEM-T Easy) so that their nucleotide sequences could be
investigated. In the RFLP analysis, the products of single and double digestion with the AfaI and HapII restriction
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
this area, sequences of the gene coding for the 18S ribosomal RNA (rRNA) of sandflies caught in a Shannon trap
were explored, by a combination of PCR and analysis of restriction-fragment-length polymorphism (RFLP). The
products from the PCR, which employed two primers developed specifically for this study (Lu.18S 1S and Lu.18S
AR), were cloned into a commercial vector (pGEM-T Easy) so that their nucleotide sequences could be
investigated. In the RFLP analysis, the products of single and double digestion with the AfaI and HapII restriction
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
Lutzomyia species in
this area, sequences of the gene coding for the 18S ribosomal RNA (rRNA) of sandflies caught in a Shannon trap
were explored, by a combination of PCR and analysis of restriction-fragment-length polymorphism (RFLP). The
products from the PCR, which employed two primers developed specifically for this study (Lu.18S 1S and Lu.18S
AR), were cloned into a commercial vector (pGEM-T Easy) so that their nucleotide sequences could be
investigated. In the RFLP analysis, the products of single and double digestion with the AfaI and HapII restriction
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
AfaI and HapII restriction
enzymes were separated by electrophoresis in 3% or 4% agarose. Taken together with the results of a morphological
investigation of the flies, the resultant DNA fragment patterns were sufficient to identify most of the sandflies
caught as Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
Lu. neivai. Although two other species, Lu. cortelezzii and Lu. sallesi, were collected, they were relatively
rare and only identified morphologically. A single digestion of the 18S-rRNA gene sequences with AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
AfaI or HapII
appeared sufficient and useful for the identification of Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.
other Lutzomyia species.
Lu. neivai from the north of Salta province, and for several
other Lutzomyia species.Lutzomyia species.