INVESTIGADORES
GONZALEZ Daniel Hector
artículos
Título:
Expression of sunflower cytochrome c mRNA is tissue-specific and controlled by nitrate and light
Autor/es:
FELITTI, SILVINA A.; CHAN, RAQUEL L.; GAGO, GABRIELA M.; VALLE, ESTELA M.; GONZALEZ, DANIEL H.
Revista:
PHYSIOLOGIA PLANTARUM
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 1997 vol. 99 p. 342 - 347
ISSN:
0031-9317
Resumen:
A PCR-based strategy with two degenerate oligonucleotides followed by
library screening was used to isolate a 590-bp cDNA clone encoding a
full-length apocytochrome c polypeptide from a sunflower (Helianthus
annuus. L. line HA401B) root library. The sequence of the encoded
polypeptide (112 amino acids) differs from the previously reported
protein sequence (Ramshaw et al. 1970. Biochem. J. 119; 535-539) at four
positions. Southern blot analysis indicates the possible existence of
two different cytochrome c genes in sunflower. The isolated cDNA clone
was used to perform expression studies of cytochrome c mRNA(s) in
different sunflower organs and under different conditions. Higher
transcript levels were observed in flowers and roots relative to leaves,
stems, hypocotyls and cotyledons. Light promoted an increase in the
expression of cytochrome c mRNA in 6-day-old seedlings. Nitrate
treatment, in turn, reduced expression in roots. It is concluded that
the steady-state mRNA levels of cytochrome c encoding transcripts in
sunflower are subject to tissue-specific-regulation and also respond to
environmental factors.