INVESTIGADORES
GONZALEZ Daniel Hector
artículos
Título:
Isolation and expression pattern of hahr 1, a homeobox-containing cDNA from Helianthus annuus
Autor/es:
VALLE, ESTELA M.; GONZALEZ, DANIEL H.; GAGO, GABRIELA M.; CHAN, RAQUEL L.
Revista:
GENE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 1997 vol. 196 p. 61 - 68
ISSN:
0378-1119
Resumen:
A 2.5 kb homeobox (HB)-containing cDNA (hahr1) was isolated from a
library prepared from rootlets of Helianthus annuus using a polymerase
chain reaction (PCR)-based strategy. The putative protein product (77
kDa) contains the homeodomain (HD) and an acidic domain at the
N-terminal region (residues 72-155). The deduced amino acid sequence of
hahr1 shares a 53% sequence identity with GLABRA2, a HD protein
associated with epidermal cell differentiation. Hahr1 expression was
primarily found in dry seeds, hypocotyls and roots at stages associated
with early developmental events. Expression was completely lacking in
leaves and flowers. Evidence for the existence of one related gene
expressed in sunflower stems was obtained by the presence of restriction
fragment length polymorphism of amplified cDNA products.