INVESTIGADORES
GONZALEZ Daniel Hector
artículos
Título:
The cytochrome c gene from the green alga Chlamydomonas reinhardtii. Structure and expression in wild-type cells and in obligate photoautotrophic (dk) mutants
Autor/es:
FELITTI, SILVINA A.; CHAN, RAQUEL L.; GONZALEZ SIERRA, MANUEL; GONZALEZ, DANIEL H.
Revista:
PLANT AND CELL PHYSIOLOGY
Editorial:
OXFORD UNIV PRESS
Referencias:
Año: 2000 vol. 41 p. 1149 - 1156
ISSN:
0032-0781
Resumen:
The expression of the Chlamydomonas reinhardtii cytochrome c gene was
studied at the steady-state mRNA level. The inclusion of acetate under
illumination produced a marked increase in cytochrome c transcripts.
This effect was not affected by two inhibitors of mitochondrial energy
metabolism. Three different obligate photoautotrophic mutants with
defective mitochondria showed normal levels of induction, suggesting
that utilization of acetate for respiration is not required for this
process. Light, in the presence or absence of acetate, also promoted an
increase in cytochrome c transcript levels. This effect could be
abolished by treatment of the cells with an inhibitor of the
photosynthetic electron transport chain, suggesting that light acts
through photosynthesis to promote the induction. In addition, a genomic
clone encompassing the Chlamydomonas cytochrome c gene has been isolated
and analyzed. The gene contains three introns, two of which are located
at positions similar to those in the rice and Arabidopsis cytochrome c
genes, indicating the existence of an evolutionary link. It is concluded
that the cytochrome c gene from C. reinhardtii is subject to metabolic
regulation through a mechanism that responds to the intracellular level
of either acetate or a compound derived from its metabolization through a
pathway different from mitochondrial respiration.