INVESTIGADORES
GONZALEZ Daniel Hector
artículos
Título:
Knotted1-like genes are strongly expressed in differentiated cell types in sunflower
Autor/es:
TIONI, MARIANA F.; GONZALEZ, DANIEL H.; CHAN, RAQUEL L.
Revista:
JOURNAL OF EXPERIMENTAL BOTANY
Editorial:
OXFORD UNIV PRESS
Referencias:
Año: 2003 vol. 54 p. 681 - 690
ISSN:
0022-0957
Resumen:
Knotted1-like genes constitute a family of genes whose products are
transcription factors involved in several aspects of plant development.
In most species, these genes are expressed primarily in meristematic
cells and are switched off as leaves develop. In this paper, the
expression patterns of three kn1-like genes from sunflower (Helianthus
annuus L.) are described. Northern blot experiments indicated that these
genes are expressed at different levels in several organs of the plant,
including flowers, leaves, stems, roots, and embryos. Notably, one of
these genes, named HAKN1, was also highly expressed in leaves and roots.
Using in situ hybridization, expression was detected in parenchymatic
cells from leaf veins, petiole and lamina, and also in stem and root.
Enhanced expression in phloem was also evident in both leaves and stem.
Another, HAKN2, showed preferential expression instem, specifically in
fascicular and interfascicular cambium and phloem. In flowers, both
genes are expressed throughout inflorescence and floral meristems and in
developing organ primordia. Strong expression of HAKN1 in developing
involucral bracts was also observed. The results show the existence of
some differencies in expression patterns of kn1-like genes in sunflower
with respect to other plants. It is proposed that cell- and
species-specific factors are involved in determining the developmental
responses of plant cells to the expression of kn1-like genes.