INVESTIGADORES
GONZALEZ Daniel Hector
artículos
Título:
Nuclear and mitochondrial genes encoding cytochrome c oxidase subunits respond differently to the same metabolic factors
Autor/es:
CURI, GRACIELA C.; WELCHEN, ELINA; CHAN, RAQUEL L.; GONZALEZ, DANIEL H.
Revista:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Año: 2003 vol. 41 p. 689 - 693
ISSN:
0981-9428
Resumen:
We have identified in the Arabidopsis thaliana Heyhn. nuclear genome a
gene encoding COX6a, a homologue of subunit 6a of the mitochondrial
cytochrome c oxidase (COX, E.C. 1.9.3.1) from animals and fungi. The
expression of this gene and of the previously identified nuclear and
mitochondrial genes, respectively, that encode subunit 6b (COX6b) and
subunit 2 of the same enzyme was analyzed by northern blot. The analysis
indicated that incubation of plants in solutions containing
metabolizable sugars produced an increase in transcript levels for the
nuclear genes but not for the mitochondrial one. The effect of
carbohydrates showed the same time-dependence for COX6a and COX6b.
Incubation of plants in solutions with different nitrogen sources also
produced changes in expression. The behavior of the nuclear genes was
very similar to that previously observed for genes encoding cytochrome c
and cytochrome oxidase subunit 5b, suggesting the operation of a common
regulatory mechanism for components of the mitochondrial respiratory
chain encoded in the nucleus, but not for their counterparts encoded in
the mitochondria. Regulation of the expression of mitochondrial genes,
if any, may operate at a different level.