INVESTIGADORES
SILVA JUNQUEIRA DE SOUZA Flavio
artículos
Título:
A P-type ATPase from the aquatic fungus Blastocladiella emersonii similar to animal Na,K-ATPases.
Autor/es:
DE SOUZA FS; GOMES SL
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA, N. GENE STRUCTURE AND EXPRESSION
Editorial:
ELSEVIER
Referencias:
Año: 1998 p. 183 - 187
ISSN:
0167-4781
Resumen:
We have cloned a P-type ATPase gene from the aquatic fungus Blastocladiella emersonii (BePAT1) using a probe obtained with degenerate oligonucleotides, corresponding to two amino acid sequences highly conserved among all P-type ATPase isoforms, and the polymerase chain reaction technique. Nucleotide sequence analysis revealed a 3.4 kb open reading frame encoding a putative peptide of 1080 amino acid residues with a calculated molecular mass of 119 kDa, which presents all diagnostic features of P-type transporting ATPases. Comparison to other members of the family and phylogenetic analyses have shown that the BePAT1 protein belongs to the subfamily of Na,K- and H,K-ATPases, indicating that the divergence between the alpha-subunit of the Na,K-ATPase and other members of the P-type ATPase family has occurred before the divergence between the animal and fungal lineages in evolution.
