Relation between cell proliferation and beta adrenergic receptor expression in activated T lymphocytes

CAZAUX CA; STERIN-BORDA L.; GORELIK G; CREMASCHI GA

ACTA PHISIOLOGICA, PHARMACOLOGICA ET THERAPEUTICA LATIONOAMERICANA

Asociacion Latinoamericana De Ciencias Fisiologicas

Lugar: Buenos Aires; Año: 1995 vol. 45 p. 43 - 48

0327-6309

beta adrenergic receptors (R) on murine lymphocytes stimulated with concanavalin A (Con A) and tumor lymphoid cell line (BW5147) were characterized by beta radioligand binding of 125Icyanopindolol (125I-CYP). The functionality was also assesses by hormone-dependent changes in cyclic 3´, 5´, adenosine monophosphate (cAMP) levels on intact cell. Both types of cells displayed a reduced number of beta adrenergic receptors by 125I-CYP specific binding. However BW5147 cell line were unable to respond to beta agonist stimulation meanwhile the response of lymphocytes stimulated with Con A was lower than normal lymphocytes. Despite this difference both kind of cells displayed an increase in cAMP production induced by Prostaglandin E1 (PGE1). It can be concluded that functional beta R are absent in BW5147 cell line. The possible implication of alternative transmission pathway and neuroendocrine control in tumor lymphoid cells is discussed.