PERSONAL DE APOYO
ASENSIO Cristian Jorge Alejandro
artículos
Título:
Outer membrane vesicles obtained from Bordetella pertussis Tohama expressing the lipid A deacylase PagL as a novel acellular vaccine candidate
Autor/es:
CRISTIAN JORGE ALEJANDRO ASENSIO; MARÍA EMILIA GAILLARD; GRISELDA MORENO; DANIELA BOTTERO; EUGENIA ZURITA; MARTÍN RUMBO; P VAN DER LEY; A VAN DER ARK; DANIELA HOZBOR
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 29 p. 1649 - 1656
ISSN:
0264-410X
Resumen:
n an effort to devise a safer and effective pertussis acelullar vaccine, outer membrane vesicles (OMVs) were engineered to decrease their endotoxicity. The pagL gene from Bordetella bronchiseptica, which encodes a lipid A 3-deacylase, was expressed in Bordetella pertussis strain Tohama I. The resulting OMVs, designated OMVs(BpPagL), contain tetra- instead of penta-acylated LOS, in addition to pertussis surface immunogens such as pertactin and pertussis toxin, as the wild type OMVs. The characterized pertussis OMVs(BpPagL) were used in murine B. pertussis intranasal (i.n.) challenge model to examine their protective capacity when delivered by i.n. routes. Immunized BALB/c mice were challenged with sublethal doses of B. pertussis. Significant differences between immunized animals and the PBS treated group were observed (p
