INVESTIGADORES
RAMOS Jorge Guillermo
artículos
Título:
Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals.
Autor/es:
DURANDO M; COCITO L; RODRÍGUEZ HA; VARAYOUD J; RAMOS JG; LUQUE EH; MUÑOZ DE TORO M
Revista:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2013 p. 31 - 38
ISSN:
0016-6480
Resumen:
Caiman latirostris is a reptilian species that exhibits
temperature-dependent sex determination (TSD). Male-to-female sex
reversal can be achieved after in ovo estrogen/xenoestrogen exposure.
This is known as hormone-dependent sex determination (HSD). The amh,
sox9 and sf-1 genes are involved in sex determination, sex
differentiation, and steroidogenesis. The aims of this study were: (a)
to establish the expression patterns of amh, sox9 and sf-1 mRNA in the
gonad-adrenal-mesonephros (GAM) complexes of neonatal TSD-male and
TSD-female caimans, (b) to compare the expression of these genes between
TSD-females and HSD-females (born from E2-exposed eggs incubated at the male-producing temperature) and (c) to evaluate whether in ovo exposure to a low dose of E2
or bisphenol A (BPA) or to a high dose of endosulfan (END) modifies
amh, sox9 or sf-1 mRNA expressions in neonatal males. The mRNA
expressions of amh, sox9 and sf-1 in GAM complexes from TSD-males and
TSD-females and from HSD-females were quantitatively compared by RT-PCR.
A sexually dimorphic pattern of amh and sox9 mRNA expression was found,
with a higher expression in TSD-males than in TSD-females. sf-1 mRNA
did not differ between TSD-males and TSD-females. HSD-females exhibited a
higher expression of sox9 than TSD-females. In males, increased mRNA
expression of sex-determining genes was observed after in ovo exposure
to END. E2 decreased sox9 but increased sf-1 mRNA expression.
Changes induced by BPA were evident although not significant. These
results provide new insights into the potential mechanisms that lead to
the gonadal histo-functional alterations observed in caimans exposed to
contaminated environments.