INVESTIGADORES
ALVAREZ IGARZABAL Cecilia Ines
artículos
Título:
Synthesis and characterization of a biospecific adsorbent containing bovine serum albumin as a ligand and its use for bilirubin retention
Autor/es:
ALVAREZ IGARZABAL C.I.; M.C. STRUMIA; HECTOR BERTORELLO
Revista:
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2001 vol. 49 p. 649 - 656
ISSN:
0165-022X
Resumen:
Epoxy-activated gels from cross-linked polybutadiene-hydroxyethylmethacrylate -PB-HEMA. copolymer and epichlorohydrin -ECH. were prepared and characterized. Albumin was covalently bonded to the matrix and used as support of affinity chromatography in bilirubin -BR. retention experiments. PB-HEMA-ECH with different amounts of immobilized albumin between 5.20 and 6.80 mgrg dry gel. were obtained. Bilirubin retention of 3.10 mgrg of these beads was observed at 5 8C.