CYTOTOXIC EFFECTS OF ACANTHOSPERMAL B ON BLOOD AND TUMOR CELLS

ARENA ME; GOBBATO N; CARTAGENA E; CARAM MM; GONZALEZ ML; BARDÓN A; VALDEZ JC

BIOCELL

INCA

Lugar: MENDOZA, ARGENTINA; Año: 2008 vol. 32 p. 95 - 95

0327-9545

The main compound of the chloroform extract of the American  herb  Acanthospermun hispidum DC, is the sesquiterpene lactone Acanthospermal B (AcB), that display bacteriolitic effects on a wild strain of Staphilococcus aureus (F7 methicillin-resistant). We investigated the effects of AcB on blood and tumor cells. Polymorphonuclear (PMN) and mononuclear (MN) cells were isolated from human blood. The tumor cells (CTM3) were obtained by enzymatic digestion from a breast cancer M3 mice Balb/c. Peritoneal macrophages (MoP) were obtained from normal mice Balb/c. Suspensions of 106 cells/ml of each kind were incubated in RPMI 1640 media plus 10% fetal bovine serum and gentamicine 100ug/ml of AcB, for 12 h at 37°C in presence of 0 (control), 10, 50 and 100 mg/ml of AcB. The viability of the human PMN and MN was determined by flow cytometry and iodure propidium and the viability of mice cells (MoP and CTM3) by trypan blue vital stain. In presence of 100 mg/ml, a decrease of 45 and 16% in the viability of PMN and MN was observed. Viability decreased in 26 and 40% in MoP and CTM3 cells, in relation to control.The highest concentration tested showed a significant but non selective cytotoxic effect on tumor and MoP mouse cells. However, the cytotoxic effect was selective for human leukocytes (PMN are more sensitive to AcB that MN). This selectivity correlates with the effect of other sesquiterpene lactones on human leukemia (HL-60). Further studies are needed to determine AcB cytotoxic effects on  different human leukemia.