INVESTIGADORES
MARCIPAR Ivan Sergio
artículos
Título:
Evaluation of recombinant antigens of Trypanosoma cruzi to diagnose infection in naturally infected dogs from Chaco region, Argentina
Autor/es:
FLORIDIA YAPUR N; VEGA BENEDETTI; MONJE RUMI MERCEDES; RAGONE PAULA; LAUTIER JUAN; TOMASINI A; DAMATO ALBERTI; LOPEZ QUIROGA I; DIOSQUE PATRICIO; MARCIPAR IVÁN; NASSER J; CIMINO R
Revista:
PARASITE IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014 vol. 36 p. 694 - 699
ISSN:
0141-9838
Resumen:
Dogs are considered the main mammal reservoir of Trypanosoma
cruzi in domiciliary environments. Consequently,
accurate detection of T. cruzi infection in canine populations
is epidemiologically relevant. Here, we analysed the utility
of the T. cruzi recombinant antigens FRA, SAPA, CP1,
Ag1 and a SAPA/TSSA VI mixture, in an ELISA format.
We used a positive control group of sera obtained from 38
dogs from the Chaco region in Argentina with positive
homogenate-ELISA reaction, all of them also positive by
xenodiagnosis and/or PCR. The negative group included 19
dogs from a nonendemic area. Sensitivity, specificity, area
under the curve (AUC) of the receiver operating charactheristic
(ROC) curve and Kappa index were obtained to compare
the diagnostic efficiency of the tests. The SAPA/TSSA
VI had the highest performance, with a sensitivity of 947%
and an AUC ROC of 099 that indicates high accuracy.
Among individual antigens, SAPA-ELISA yielded the highest
sensitivity (868%) and AUC ROC (096), whereas
FRA-ELISA was the least efficient test (sensitivity
= 368%; AUC ROC = 053). Our results showed that
the use of SAPA/TSSA VI in ELISAs could be a useful tool
to study dogs naturally infected with T. cruzi in endemic
areas.