A DNA vaccine coding for the Brucella Outer Membrane Protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response

J. CASSATARO, A. VELIKOVSKY, SILVIA DE LA BARRERA, SILVIA M. ESTEIN, L. BRUNO, RAÚL BOWDEN, K. PASQUEVICH, CARLOS A. FOSSATI, GUILLERMO H. GIAMBARTOLOMEI.

INFECTION AND IMMUNITY

American Association of Veterinary Laboratory Diagnosticians

Año: 2005 vol. 73 p. 6537 - 6546

0019-9567

The development of an effective subunit vaccine against brucellosis is a research area of intense interest. The outer membrane proteins (Omps) of Brucella spp. have been extensively characterized as potential immunogenic and protective antigens. This study was conducted to evaluate the immunogenicity and protective efficacy of the B. melitensis Omp31 gene cloned in the pCI plasmid (pCIOmp31). Immunization of BALB/c mice with pCIOmp31 conferred protection against B. ovis and B. melitensis infection. Mice vaccinated with pCIOmp31 developed a very weak humoral response, and in vitro stimulation of their splenocytes with recombinant Omp31 did not induced the secretion of gamma interferon. Splenocytes from Omp31-vaccinated animals induced a specific cytotoxic-T-lymphocyte activity, which leads to the in vitro lysis of Brucella-infected macrophages. pCIOmp31 immunization elicited mainly CD8_ T cells, which mediate cytotoxicity via perforins, but also CD4_ T cells, which mediate lysis via the Fas-FasL pathway. In vivo depletion of T-cell subsets showed that the pCIOmp31-induced protection against Brucella infection is mediated predominantly by CD8_ T cells, although CD4_T cells also contribute. Our results demonstrate that the Omp31 DNA vaccine induces cytotoxic responses that have the potential to contribute to protection against Brucella infection. The protective response could be related to the induction of CD8_ T cells that eliminate Brucella-infected cells via the perforin pathway.