INVESTIGADORES
HOZBOR Daniela Flavia
artículos
Título:
Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles
Autor/es:
ORMAZABAL M; BARTEL E; GAILLARD ME; BOTTERO D; ERREA A; ZURITA E; MORENO G; RUMBO M; CASTUMA C; FLORES D; MARTIN, M; HOZBOR D
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 32 p. 6084 - 6090
ISSN:
0264-410X
Resumen:
Pertussis has resurged during the last two decades in different
countries. In particular in the 2010-2013 period large outbreaks were
detected in US, Australia, UK and The Netherlands with significant
mortality in infants. The epidemiological situation of pertussis points
out the need to develop new vaccines and in this regard we previously
developed a new vaccine based on outer membrane vesicles (OMVs) which
have been shown to be safe and to induce protection in mice. Here we
have further investigated the properties of OMVs vaccines; in particular
we studied the contribution of pertussis toxin (PTx) and pertactin
(Prn) in OMVs-mediated protection against pertussis. PTx-deficient OMVs
and Prn-deficient OMVs were obtained from defective Bordetella pertussis
mutants. The absence of PTx or Prn did compromise the protective
capacity of the OMVs formulated as Tdap vaccine. Whereas the protective
efficacy of the PTx-deficient OMVs in mice was comparable to
Prn-deficient OMVs, the protective capacity of both of them was
significantly impaired when it was compared with the wild type OMVs.
Interestingly, using OMVs obtained from a B. pertussis strain which does
not express any of the virulence factors but expresses the avirulent
phenotype; we observed that the protective ability of such OMVs was
lower than that of OMVs obtained from virulent B. pertussis phase.
However, it was surprising that although the protective capacity of
avirulent OMVs was lower, they were still protective in the used mice
model. These results allow us to hypothesize that OMVs from avirulent
phase shares protective components with all OMVs assayed. Using an
immune proteomic strategy we identified some common components that
could play an important role in protection against pertussis.