Interaction of chagasic autoantibodies with the third extracellular domain of the human heart muscarinic receptor. Functional and pathological implicationsdominio extracelular del receptor muscarínico humano

GOIN, J.C.; PÉREZ LEIRÓS, C.; BORDA, E.; STERIN-BORDA, L.

MEDICINA (BUENOS AIRES)

Fundación Revista Medicina

Lugar: Buenos Aires; Año: 1996 vol. 56 p. 699 - 704

0025-7680

Herein we demonstrate by ELISA and immunoblotting the presence in the sera of chagasic patients of circulating autoantibodies against the third extracellular domain of human muscarinic acetylcholine receptors by using a synthetic peptide corresponding to the sequence 169-192 of the receptor. Immunoaffinity purified antipeptide antibodies displayed cardiac muscarinic activity as decreased contractility and cAMP production and increased cGMP levels. These effects were specifically blocked by the synthetic peptide and by atropine. A strong association between the existence of circulating autoantibodies and the presence of dysautonomia was shown, making these autoantibodies an appropriate marker of heart autonomic dysfunction.