The nitric oxide synthase of mouse spermatozoa

HERRERO, M.B.; GOIN, J.C.; BOQUET, M.; CANTEROS, M.G.; FRANCHI, A.M.; PEREZ MARTINEZ, S.; POLAK, J.M.; VIGGIANO, J.M.; GIMENO, M.A.

FEBS LETTERS

Elsevier Science B.V.

Lugar: Amsterdam; Año: 1997 vol. 411 p. 39 - 42

0014-5793

Nitric oxide synthase (NOS) was evidenced in mature mouse spermatozoa by means of biochemical techniques and Western blot. During 120 min of incubation, 10(7) spermatozoa synthesized 7 +/- 2 pmol of L-[14C]citrulline. Besides, L-citrulline formation depended on the incubation time and on the concentration of L-arginine present in the incubation medium. Different concentrations of N(G)-nitro-L-arginine methyl ester (L-NAME) but not aminoguanidine, inhibited L-[14C]citrulline formation. Western-blot analysis of solubilized sperm proteins revealed a unique band of M(r)=140 kDa with the neural, endothelial and inducible NOS antisera tested. These results provide evidence that mature mouse sperm contains a NOS isoform and that spermatozoa have the potential ability to synthesize NO, suggesting a role for endogenous NO on mammalian sperm function.