INVESTIGADORES
LEVIN Laura Noemi
artículos
Título:
EVALUACIÓN DE ACTIVIDADES ENDOGLUCANASA, EXOGLUCANASA, LACASA Y LIGNINA PEROXIDASA EN DIEZ HONGOS DE PUDRICIÓN BLANCA
Autor/es:
SANDRA MONTOYA B; ÓSCAR JULIÁN SÁNCHEZ T; LAURA LEVIN
Revista:
Biotecnología en el Sector Agropecuario y Agroindustrial
Editorial:
Universidad del Cauca
Referencias:
Lugar: Popayán; Año: 2014 vol. 12 p. 115 - 124
ISSN:
1909-9959
Resumen:
This paper presents a way of tracking the production of lignocellulolytic
enzymes in ten species of white rot fungi: Lentinula edodes, Schizophyllum
commune, Trametes trogii, Coriolus versicolor, Pycnoporus sanguineus,
Ganoderma applanatum, Ganoderma lucidum, Grifola frondosa, Pleurotus
ostreatus and Auricularia delicata. These species were first screened on
solid culture media containing carboxymethyl cellulose, crystalline cellulose,
ABTS (2,2´-azino-bis(3-ethylbenzothiazoline-6-sulphonate)) and azure
B, which showed the production of endoglucanase, exoglucanase, laccase
and lignin peroxidase (LiP) enzymes. Cellulolytic activities were detected
after five days of incubation with congo red indicator, forming a clear-white
halo in areas where cellulose was degraded. For ligninases, the tracking
consisted of the monitoring in the formation of green halos due to ABTS
oxidation for laccase, and decolorization halos on azure B for LiP during
14 days of incubation. From this qualitative screening, four strains were
selected (G. lucidum, L. edodes, C. versicolor and T. trogii) as the best
producers of cellulolytic and ligninolytic enzymes. These four species were
inoculated on a substrate of sawdust oak, yielding 51,8% of lignin degraded
by L. edodes and 22% of cellulose degraded by C. versicolor.