INVESTIGADORES
GIUSTI Sebastian Alejandro
artículos
Título:
MicroRNA-9 controls dendritic development by targeting REST
Autor/es:
SEBASTIAN A. GIUSTI; ANNETTE M. VOGL; MARISA M. BROCKMANN; CLAUDIA A. VERCELLI; MARTIN L. REIN; DIETRICH TRUEMBACH; WOLFGANG WURST; DEMIAN CAZALLA; VALENTIN STEIN; JAN M. DEUSSING; DAMIAN REFOJO
Revista:
eLIFE
Editorial:
eLife Sciences Publications, Ltd
Referencias:
Año: 2014
ISSN:
2050-084X
Resumen:
MicroRNAs (miRNAs)
are conserved noncoding RNAs that function as posttranscriptional regulators of
gene expression. MiR-9 is one of the most abundant miRNAs in the brain.
Although the function of miR-9 has been well-characterized in neural progenitors,
its role in dendritic and synaptic development remains largely unknown. In
order to target miR-9 in vivo, we developed
a transgenic miRNA-sponge mouse line allowing
conditional inactivation of the miR-9 family in a spatio-temporal-controlled
manner. Using this novel approach we found that miR-9 controls dendritic
growth and synaptic transmission in vivo.
Furthermore, we demonstrate that miR-9-mediated downregulation of the
transcriptional repressor REST is essential for proper dendritic growth.